Saccharomyces cerevisiae signature genes for predicting nitrogen deficiency during alcoholic fermentation.
نویسندگان
چکیده
Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. Reverse transcription-PCR analysis for four of these transcripts with this strain and its validation with another wine yeast strain underlines the usefulness of these signature genes for predicting nitrogen deficiency and therefore the diagnosis of wine stuck/sluggish fermentations.
منابع مشابه
Transcriptional response of Saccharomyces cerevisiae to different nitrogen concentrations during alcoholic fermentation.
Gene expression profiles of a wine strain of Saccharomyces cerevisiae PYCC4072 were monitored during alcoholic fermentations with three different nitrogen supplies: (i) control fermentation (with enough nitrogen to complete sugar fermentation), (ii) nitrogen-limiting fermentation, and (iii) the addition of nitrogen to the nitrogen-limiting fermentation (refed fermentation). Approximately 70% of...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 73 16 شماره
صفحات -
تاریخ انتشار 2007